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We excel at flow cytometry, it's what we do!

We provide flow cytometry testing services in support of clinical trials, nonclinical studies, toxicology assessments, research and development and the evaluation of flow cytometric products.

We pride ourselves on the ability to customize any flow cytometry assay to meet our client's needs. We also have extensive experience in data collection and analysis utilizing both straight-forward and complex gating strategies.

While we specialize in assay development and customization, we also welcome routine flow cytometry projects such as standard surface immunophenotyping.

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News and Information

  • Latest News
  • Upcoming Events
  • Company Literature
  • AAPS Flow Cytometry APC
  • Job Openings
About FCS Laboratory

About FCS Laboratory

  • About Us
  • Leadership
  • Specialized Service
  • Specimen Shipping
  • Contact Us
Services

Our Flow Cytometry Services

  • Immunophenotyping
  • Bead-Based Methods
  • Cell Signaling
  • Cellular Functional Assays
  • Consulting Services

FCS image

We excel at flow cytometry, it's what we do!

We provide flow cytometry testing services in support of clinical trials, nonclinical studies, toxicology assessments, research and development and the evaluation of flow cytometric products.

We pride ourselves on the ability to customize any flow cytometry assay to meet our client's needs. We also have extensive experience in data collection and analysis utilizing both straight-forward and complex gating strategies.

While we specialize in assay development and customization, we also welcome routine flow cytometry projects such as standard surface immunophenotyping.

Artwork by Abu Atienza and Logo by Christian Tan.

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Latest News

We are excited to announce the addition of another BD FACSCantoIITM to our collection of flow cytometers. With this instrument, we now have three flow cytometers that are available to meet our client's needs.

Our manuscript "Recommendations for the Evaluation of Specimen Stability for Flow Cytometric Testing During Drug Development" has been recently accepted by the Journal of Immunological Methods and is now available online.

We currently have two manuscripts submitted to a special Receptor Occupancy issue of Cytometry Part B: Cinical Cytometry.

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Relevant upcoming scheduled events (meetings, conferences, seminars, symposia etc.):

Event/Conference Date Location
SOT Annual Meeting and Tox Expo Mar 22-26th, 2015 San Diego, CA
AAPS National Biotechnology Conference Jun 8-10th, 2015 San Francisco, CA
ISAC CYTO 2015 Jun 26-30th, 2015 Glasgow, Scotland
Annual Clinical Cytometry Meeting Oct 9-13th, 2015 Denver, CO
AAPS Annual Meeting Oct 25-29th, 2015 Orlando, FL
American College of Toxicology Meeting Nov 8-11th, 2015 Summerlin, NV

If you are interested in scheduling a time to meet with a FCS Laboratory representative to learn more about our services and capabilities or to discuss how we can assist you with your nonclinical and clinical flow cytometry needs, please contact us at (425) 821-3900 or email: info@fcslaboratory.com.

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Our Mission

"We collaborate with our clients, respond to their needs, anticipate regulatory change, invest in our facilities and our people and maintain ourselves at the forefront of flow cytometry."

Our Vision

"Just name it, we can do it!"

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Literature

For more information please see the resources listed below:

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Current Job Openings

FCS Laboratory is building a team of high-caliber scientists who believe in the potential of very specialized testing. We are still a small start-up where individual contributions are highly valued and recognized. In our fast-paced environment, we are helping our clients develop life-changing products and therapies.

    We are currently not hiring for any positions. Please check back in the future as this may change from time to time.

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Brochures and Flyers

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Peer-Reviewed Journal Articles

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The Marker Archive

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  • 2011
  • 2012
  • 2013
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    The Marker Archive

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    Flow Cytometry Action Programming Committee

    The FCAPC is a subgroup of the American Association of Pharmaceutical Scientists (AAPS) Ligand Binding Assay Bioanalytical Focus Group (LBABFG). We are proud members of this collaboration of experts, with the main goal being to establish recommendations, guidelines and criteria for using flow cytometry assays and instrumentation during drug development.

    Please visit our new interactive LinkedIn Group "Flow Cytometry Action Program Committee" for more information or to join.

    Stay tuned for future FCAPC programs!

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    About Us

      Founded in 2009, FCS Laboratory specializes in flow cytometry services for drug development. We provide:

    • First rate and personal customer service with frequent project updates
    • State of the art facilities and equipment
    • Prompt, clear, comprehensive, and accurate reporting
    • Turn-around time unequaled by other contract laboratories
    • Up front itemized pricing with no hidden costs
    • Expertise in client processes, eliminating turnover issues
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    The FCS Laboratory Story

    Contact FCS Laboratory

    FCS Laboratory is located in the Totem Valley Business Center in Kirkland, WA (approximately 30 min east of Seattle).

    Flow Contract Site Laboratory, LLC

    13029 NE 126th Place, Unit A229

    Kirkland, WA 98034-7723

    Phone: (425) 821-3900

    FAX: (425) 821-3925

    Email: info@fcslaboratory.com

    To join our mailing list please email marker@fcslaboratory.com.

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    Leadership

    The owners have combined experience of 20+ years in flow cytometry and 15+ years working in a Good Laboratory Practice (GLP) environment.

    The Management Team

    Lynette Brown, President and Flow Cytometry Specialist

    Jennifer J. Stewart, Vice President and Laboratory Director

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    lynette brown

    Lynette Brown, MS, CHT

    Lynette has 10 years professional experience in flow cytometry both in the clinical and research settings. Lynette has experience with many specimen types including whole blood and tissue from rodents, dogs, non-human primates and humans. She has developed and validated numerous assays for surface and intracellular markers and NK cell function that have been used on more than 50 GLP studies.

    Lynette is in charge of the day to day operation of the laboratory which includes training, instrument maintenance and validation, specimen handling, sample acquisition and data analysis.

    View Lynette Brown's profile on LinkedIn

    jennifer stewart

    Jennifer J Stewart, PhD

    Dr. Stewart has over 20 years professional experience and is responsible for overseeing internal and client sponsored research, preclinical or clinical drug studies using human specimens, non-human primate, dog or rodent models. She scientifically reviews the documentation, analyzes and reports flow cytometry data and communicates directly with Study Directors, Study Monitors and Clients.

    Dr. Stewart is currently serving as a guest editor for Cytometry Part B: Clinical Cytometry and she is a scientific reviewer for the Journal of Immunological Methods and Clinical and Vaccine Immunology.

    View Jennifer J. Stewart, PhD's profile on LinkedIn

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    Specialized Service

    By focusing on flow cytometry services, FCS Laboratory is able to ensure that there is no dilution of the scientific and managerial focus because of unrelated upstream and downstream functions.

    We utilize Becton Dickinson flow cytometers. Currently our facility houses two BD FACSCantoII cytometers with two scatter parameters and up to 8 flourescent channels and a BD FACSCantoA cytometer with two scatter parameters and up to 6 flourescent channels. We also have a Guava PCA which performs all of our cell count and viability assessments.

    We are proficient in processing a multitude of specimen types including whole blood, tissue, frozen PBMCs and tumors for pre-clinical model systems and human clinical trials.

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    Specimen Shipping

    FCS Laboratory operates as an outsourcing CRO in which specimens are shipped to us for analysis. This provides our clients more flexibility in choosing a laboratory and can reduce overload of their internal resources.

    Since we rely on shipped specimens, we conduct rigorous qualification and validation of our assays to ensure specimen stability is well understood. We will work closely with you to determine appropriate sample preparation and shipping requirements for a particular assay.

    Please see our poster showing the stability of surface, activation and intracellular markers on shipped specimens under US domestic and international shipping time frames.

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    Immunophenotyping

    We use BD FACSCantoTM technology to analyze up to 8-color antibody panels. Our instruments are validated to meet GLP and 21 CFR Part 11 compliance.

      Please see our flyer for more detailed information on our antibody panels.

    • T, B and NK cell enumeration
    • T-lymphocytes and subsets, B-lymphocytes and subsets
    • Monocytes, Granulocytes and Dendritic Cells
    • Receptor Occupancy Assays
    • Custom Panels also available, please inquire!
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    Bead-Based Methods

    We utilize commercially available bead sets to evaluate the following:

    • HLA typing using One Lamda's FlowPRA® technology
    • Excreted cytokines with the BDTM Cytometric Bead Array (CBA) Kits and the Ebiosciences FlowCytomixTM Multiple Analyte Detection System
    • Enrichment of cell populations of interest using STEMCELL Technologies EasySep® and Miltenyi MACS® Separators
    • Enumeration of absolute lymphocyte counts using counting particles. Please see our poster for more info.
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    Cell Signaling

    We are proud to offer flow cytometry assays for elucidation of signal transduction pathways.

      Please see our flyer for more detailed information on our intracellular markers and stains.

    • Intracellular protein staining of cytokines and transcription factors
    • Detection of key protein activation states and cross-talk using phospho-specific antibodies
    • Measurements of DNA content, Cell Cycle Progression and Apoptosis
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    Cellular Functional Assays

    We have full service cell culture capabilities to demonstrate whether or not a cell has the capacity of performing certain functions.

    • Cell count and viability measurements by Guava® PCATM technology
    • Cell Proliferation Assay
    • CTL Assay
    • Natural Killer (NK) Cell Activity, please see our poster for more information on correlation of surface biomarker expression with NK Cell killing
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    Consulting Services

    In addition to our full service flow cytometry offerings, we are pleased to add the following consulting services.

    • Support flow cytometry laboratory set-up and operations
    • Assist with assay development and troubleshooting
    • Facilitate assay transfer from originating laboratory to the site of testing
    • Oversee flow cytometry portions of projects or studies on a global scale
    • Analysis of existing FCS files
    • Data interpretation and reporting
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    We want to hear from you!

    Please direct inquiries and/or questions to info@fcslaboratory.com. To help us respond to your request, please enter your name, email address, phone number, company name and a brief description of your flow cytometry needs and a representative from FCS Laboratory will respond to you shortly.

    For a faster response and to speak directly to a flow cytometry expert, do not hesitate to call us at (425) 821-3900.

    Thank you for your interest in FCS Laboratory!

    To join our mailing list and receive our quarterly updates, please email marker@fcslaboratory.com.

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